常見載體引物表

2023年12月4日發(fā)(作者：沐浴春風)

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常見載體引物表

Vector

PBAD

pACT

pACT2

pAS2-1

pB42AD

pBACPAK8

pBK-CMV

PBS(SK/KS)/M13-

pBV220

pCAMBIA 1301(1300)

pCAMBIA 2300

PCANTB5E

pCAT3-enhancer

pcDNA4

pcDNA6

pcDNAII

pCEP4

pCF-T

pCI

pCI-neo

pCMS-EGFP

pCMV-3Tag-4A

pCMV5

pCMV5-Flag

pCMV-MYC/NUC

PCMV-HA

pCMV-Sport

pCMV-Tag(KAN+)

pCS2

pDonar

pDONR221

pDrive

pDRIveR(KAN+)

pDsRED1-C1

pDsRed1-N1

pDsRed-Monomer-N1

pDsRED2-C1(KAN+)

pDSRED-N1(KAN+)

Primer(F)

PBAD-F/TRXF

T7

Ac5-Profor

GAL4-AD-Cfor

GAL4 BD

pB42ADF

BAC1

T7

M13F/T7

PBV220F

P1

M13R(-48)

S1/M13R

RVP3

CMV-F/T7

CMV-F/T7

T7/CMV-F

Primer(R)

PBAD-R

T3

BGH

pGAL4-ADrv

pB42ADR

BAC2

T3

T3/M13R

PBV220R

P2〔同2.0-rev〕

M13F(-47)

S6

此載體無反向引物

SP6/BGH

BGH

BGH

T7/CMV-F(J21025在T7前面〕

BGH

T7 SP6

M13F M13R

pCEP-F EBV-R

M13F M13R

T7〔17Ba〕 此載體無反向引物

T7〔17Ba〕 T3

T7 T3

T3 /PFLAG-CMV-F T7

pCMV5F pCMV5R

pCMV5F pCMV5R

CMV-F BGH

CMV-F EBV-R

M13F/T7 SP6/M13R

T3 T7

M13F/T7 M13R

T7 BGH

SP6 T7

M13F M13R

M13F M13R

T7 SP6

T7 SP6

pEGFP-N-3’(距離很近,一般不pDsRED-ex-C1-F

CMV-Profor

CMV-Profor

用)

RFP-Nrev

DSRED1_n_primer

pDsRED-ex-C1-F

pEGFP-N-5’

pEGFP-N-3’

PDSRED-N-R pECFP-C

pECFP-N1

pEF/myc/ER

pEGFP-C(KAN+)

pEGFP-N(KAN+)

pENTR/D-TOPO(KAN+)

pET-*(His)(KAN+)

pET22(a,b,c)(AMP+)

pET28,30,24,49(KAN+)

pET32(a,b,c)(AMP+)

pET-42a (-b, -c)(+)

pET50(amp+)

pET44(AMP+)

pEYFP-N1

PETBlue

pEYFP-C1

pFLAG-CMV

pGAD424

pGADGH

pGADT7-Rec

pGAPZa-A

pGBKT7(Kana+)

pGEM-T(-Easy)

pGEX-(*)T

pGL2

pGL3

PinpointTM Vector

pIRES

pIRES2-EGFP

pLXSN

pMAL-c2E

pMAL-C2x

pMAL-p2X

PMD18-T

PMD19-T

pPIC9K(AMP+、ZEO+〕

pPICZa

pPROEXHTA

pQE30or40

pRSET

pSilencer3.1-H1 hygro

pSK01-T

pSK-CMV(KAN+)

pSP72

pSport1

pSUPER

pTA2

pT-Adv

pEGFP-C-5’

pEGFP-N-5’

pEF-F

pEGFP-C-5’

pEGFP-N-5’

M13F

T7

T7

T7

Stag

pEGFP-N-5’

PETBlueT7UP(太靠前，在T7前)/ PETBlueUP

pEGFP-C-5’

CMV30

GAL4 AD

GAL4 AD

GAL4 AD/T7

a-FACTOR

T7

M13F/T7

pGEX-4T-5’

GLP1

RVP3

Pinpoint Primer

PIRES-F/T7

5’

pLXSN-F

MalE primer

P5’

MalE primer

M13R(-48)

M13F(-47)

5’AOX/a-factor

5'AOX/PPICZa-F

M13R(-48)

pQE30-F

T7

M13F(-47)

T7

M13F

T7

T7

SP6/M13F(-47)

T7

M13F/T7(優(yōu)先使用M13F〕

M13F /T7

pECFP-C-3'

pEGFP-N-3’

pEGFP-C-3'

pEGFP-N-3’

M13R

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7 Ter

T7TER

T7 Ter

pEGFP-N-3’

PETBlueDOWN

pEGFP-C-3'

CMV-24

3'AD

3'AD

3'AD

3'AOX

3'BD

SP6/M13R

pGEX-4T-3’

GLP2

GLP2(RVP4)

SP6

T3/PIRES-R (J40720)

3’

pLXSN-R

M13F(-47)

P3’

M13F(-47)

M13F(-47)

M13R(-48)

3’AOX

3'AOX

無反向引物

pQE-R:

T7TER

M13R

T3

SP6

T7/M13R

T3

T3/M13R

M13R pTARGET TM

pTO-T7

pTRC99a-c

pTriPLEx2

pTWIN-1

pUC18(19)/118(119)

pUC57

pUCm-T

pVAX1

pWSK29

pXT7

pYES2

pLEXA

pLNCX

pRECEIVER

pSHUTTLE-CMV

pSP64/65

pSTBlue-1

pT7Blue(R)

pRK5

pMSCV

pBABE

pFastBac HT_A (_B, _C)

pFastBac1

pACYCDuet-1 (MCS I)

pDSRED2-N1(KAN+)

pFastBac Dual (MCSI)

pBSN-T

pHISi－1

pRSV-Rev(根據(jù)酶切位點選擇不同引物)

EZ-T

pMSCV-hyg

PTHIOHIS (A )

PDEST22

pLXSN

pLEXA

pCDH

PVX

pAcGP67 (_A, _B, _C)

pAD-Track

PLPC

pHAGE-CMV-MCS-IZsGreen

PLENTI-HIS

pDSRED-PRESS-C1

pCR-Blunt

pMT/V5-His_A (_B,_C)

pEASY-T3

pTarget.F(在T7前面〕/T7 M13R

T7

pTRC99C-F

5'pTriPLEx2

T7

M13F(-47)

M13R(-48)/M13R

M13F/T7

T7

T7/M13F/M13F(-47)

T7

T7

pLEXA-F

pLNCX-F

CMV-F

PSHUTTLE-CMV-F

SP6

T7

T7

SP6

MSCV5'

pBABE5’

pFastBac-F

PH-Profor/BaculoVfor

T7TER

pTRC99C-R

T7

T7TER(客戶確認再用〕

M13R(-48)

M13F(-47)/M13F

M13R

BGH

T3/M13R/M13R(-48)

SP6

pLEXA-R

pLNCX-R

此載體無反向引物

PSHUTTLE-CMV-R

此載體無反向引物

SP6

M13F(-47)

pSG5-3' or SV40pA-R

MSCV-rev

pBABE3’

pFastBac-R

pFastBac-R

DuetDOWN1

DsRed1-N

pFastBac-R/SV40-pArev

T3

無

ACYCDuetUP 1

pEGFP-N-5

BaculoVfor/PH

T7

PHISi-1

pREPF/M13F

HIV-Rev-NES/pGEX-3'

M13F(-47)

MSCV5'

TRXF(new)

PDEST22-F

pLXSNfw

pLEXA-F

pCDH-EF1

LBA

PH

pAD-Track-F

PLPC-F/CMV-F

pHAGE-CMV-F

CMV-F

pDSRED -C1-F

M13R

MT-Profor

T7/M13F

T7

M13R(-48)

PMSCV-R/ MSCV-rev

M13F

PDEST22-R

pLXSNrv

pLEXA-R

無

LBB

pAcGP67rv

無

PLPC-R

暫沒設計

無

pDSRED-C1-R

M13F/T7

BGHrev

SP6/M13R

2.0REV(同P2(pCAMBIA 1301)) pLVX-IRES-ZsGreen1 PIRES2-EGFP-F PIRES2-EGFP-R

pDC316

PVAX1

PSHUTTLE-CMV

pSilencer? 4.1-CMV neo

pDC316F

PLXSN-5

T7/CMV-F

pShuttle-CMV-F

pDC316R

PLXSN-3

BGH

pShuttle-CMV-R

P2(pCAMBIA 1301)

M13F

PSI-CHECK2-F

CMV-F

PSICHECF-2-R1

pMIR-report

PSI-CHECK2

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