中國油料作物學報Chine Journal of Oil Crop Sciences 水分脅迫下叢枝菌根真菌對澳洲堅果幼苗
磷利用效率的影響
岳海，何雙凌，耿建建，陶亮，倪書邦，賀熙勇*
（云南省熱帶作物科學研究所，云南景洪，666100）
摘要：基于云南熱區澳洲堅果種植區季節性干旱及先天性低有效磷的土壤特征，探討叢枝菌根真菌（arbuscular
mycorrhizal fungi ，AMF ）接種對具有排根的引種作物-澳洲堅果幼苗磷利用的影響，為外來物種的本地馴化栽培提供理論依據。在正常水分與水分脅迫條件下，對盆栽的澳洲堅果幼苗分別接種土著叢枝菌根真菌（native arbuscu?lar mycorrhizal fungi ）和摩西球囊霉（Glomus mosae ），測定接種不同AMF 對澳洲堅果幼苗生長、菌根定殖、酸性磷酸酶活性、檸檬酸分泌及磷素吸收等的影響。結果表明，接種土著AMF （N-AM ）處理對澳洲堅果幼苗排根或非排根的侵染率顯著高于接種摩西球囊霉（G-AM ）處理，正常水分條件下N-AM 處理的侵染率最高；兩種水分條件下，接種AMF 的植株地上部和地下部干重更高，正常水分處理下達到最大。菌根侵染率與排根產生量之間顯著正相關，水分脅迫顯著抑制了植株的排根產生量；與不接種相比，接種處理能顯著提高排根產生量。接種AMF 后根系對磷素的活化、吸收及轉化能力顯著升高，表現為接種的澳洲堅果幼苗根系、莖稈和葉片中全磷含量較高，兩種水分條件
下均表現為N-AM>G-AM>CK ；菌根與排根相互協調共同發揮作用，顯著改善了澳洲堅果幼苗根系對磷素的活化和吸收能力，且菌根比排根發揮了更大作用，尤其在水分脅迫條件下。兩種AMF 均能與澳洲堅果幼苗建立共生關系，N-AM 處理的幼苗各指標顯著優于G-AM 處理，可能是由于擁有多樣性優勢的土著AMF 在澳洲堅果幼苗根系中定殖能力較強或是AMF 真菌對外來物種澳洲堅果有正反饋作用。
關鍵詞：澳洲堅果；叢枝菌根真菌；排根；磷利用
中圖分類號：S664.9文獻標識碼：A 文章編號：1007-9084（2020）02-0285-07
Effect of colonization with arbuscular mycorrhizal fungi on phosphorus utilization of macadamia edlings
under water stress YUE Hai ，HE Shuang-lin ，Geng Jian-jian ，TAO Liang ，NI Shu-bang ，HE Xi-yong *
（Yunnan Institute of Tropical Crops,Jinghong 666100,China ）
Abstract:The effects of arbuscular mycorrhizal fungi (AMF)inoculation on drought tolerance in macadamia edlings with cluster rootss,were studied bad on asonal drought and congenital lo
w phosphorus soil in Yunnan tropical regions,providing a theoretical basis for domestication and cultivation of alien species.An experiment of potted macadamia edlings with inoculated Native-AMF and Glomus mosae under well-watered or water stress condition was conducted to determine the growth,AMF colonization,acid phosphata activity,cretion of citric and phosphorus utilization,etc.The colonization rate of N-AM treatment on edlings with cluster roots and without cluster roots were significantly higher than that of under G-AM treatment,and it would reach the maximum under well-watered condition.Compared with control,the plant with AMF had higher dry biomass in shoots and roots in both treatments,and it would perform best in well-watered condition.There was a significant positive correlation be?tween AMF colonization rate and number of cluster roots.The water stress significantly inhibited the production of cluster roots,but the inoculation promoted cluster roots number.Macadamia edlings had high total phosphorus content in roots,stems and leaves after inoculated with AMF,which enhanced the root ability to activate,absorb and 2020，42（2）：285-291doi ：10.19802/j.issn.1007-9084.2019200
收稿日期：2019?08?07
基金項目：南亞熱作技術試驗示范與服務支持（151721301064072712）；云南省熱帶作物科技創新體系建設專項（RF2017-427-8）作者簡介：岳海（1976-），男，副研究員，主要研究方向為熱帶果
樹生理生態，E-mail ：yuehaiwzh@126 *通訊作者：賀熙勇（1973-），男，湖南人，研究員，主要研究方向為果樹學，E-mail ：heda0691@sina OSID
